Part 1: First we need to create our gel.
Part 2: Next, we need to load and run our gel.
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Procedure: Confirm the actual species of origin for our samples and compare it to the putative species (what is was sold as).
1. View the resulting sequences for each of our samples HERE. 2. You are assigned to determine the species corresponding to your group number in lab. View the putative species for each sample HERE.
3. Visit the National Center for Biotechnology Information (NCBI) database called GenBank. 4. Follow the steps in the slide show below to match your sequence to all those stored in GenBank. Start at THIS LINK: The Genebank BLAST Page. 5. Once you've identified the samples' species of origin, add it to your data table along with the putative species. |
Lab will precede in three parts.
1) Gel electrophoresis: You will create an agarose gel in lab. You will place a small amount of the PCR product from each bushmeat sample into the gel. If we synthesized the correct DNA, all our samples should be about 490 base pairs long. You will view these results to determine if our PCR was successful. 2) Analyze the sequence results: You will be provided with output from the DNA sequencer in the WKU Biotechnology Center for each bushmeat sample. You will then enter the sequences into a database for species identification. 3) Conclusions: Lastly, we will discuss the bushmeat crisis as a class. |
Procedure: Confirm the actual species of origin for our samples and compare it to the putative species (what is was sold as).
1. View the resulting sequences for each of our samples HERE. 2. You are assigned to determine the species corresponding to your group number in lab. View the putative species for each sample HERE.
3. Visit the National Center for Biotechnology Information (NCBI) database called GenBank. 4. Follow the steps in the slide show below to match your sequence to all those stored in GenBank. Start at THIS LINK: The Genebank BLAST Page. 5. Once you've identified the samples' species of origin, add it to your data table along with the putative species. |